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KMID : 1044820230530040248
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Journal of Periodontal & Implant Science
2023 Volume.53 No. 4 p.248 ~ p.258
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Temporal changes of periodontal tissue pathology in a periodontitis animal model
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Yoon Hyun-Pil
Jung Bo-Hyun
Yoo Ki-Yeon
Lee Jong-Bin
Um Heung-Sik
Chang Beom-Seok
Lee Jae-Kwan
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Abstract
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Purpose: This study aimed to characterize the early stages of periodontal disease and determine the optimal period for its evaluation in a mouse model. The association between the duration of ligation and its effect on the dentogingival area in mice was evaluated using micro-computed tomography (CT) and histological analysis.
Methods: Ninety mice were allocated to an untreated control group or a ligation group in which periodontitis was induced by a 6-0 silk ligation around the left second maxillary molar.
Mice were sacrificed at 1, 2, 3, 4, 5, 8, 11, and 14 days after ligature placement. Alveolar bone destruction was evaluated using micro-CT. Histological analysis was performed to assess the immune-inflammatory processes in the periodontal tissue.
Results: No significant difference in alveolar bone loss was found compared to the control group until day 3 after ligature placement, and a gradual increase in alveolar bone loss was observed from 4 to 8 days following ligature placement. No significant between-group differences were observed after 8 days. The histological analysis demonstrated that the inflammatory response was evident from day 4.
Conclusions: Our findings in a mouse model provide experimental evidence that ligatureinduced periodontitis models offer a consistent progression of disease with marginal attachment down-growth, inflammatory infiltration, and alveolar bone loss.
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KEYWORD
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Histological techniques, Ligation, Mice, Periodontal diseases, Silk, X-ray microtomography
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